Bio Gene is one of the only two manufacturers in the world for Gene Gun System. We supply desk-top and hand-held gene gun system to our domestic customers over ten years. Applying Gene Gun system, many top laboratories have used for research purpose & Gene Transformation research with impressive results. Many important thesis have been published in the professional magazines home and abroad. Using our Gene Gun.
The Gene gun provides a completely new technique for shooting genetic material and other substances into cells, tissues and organ cells by high-velocity micro-projectiles. And it is the latest gene-transferred technology. Driven by the pressure of compressed gas (He or N2 etc.), the gene gun generates a cold gas shock wave into the bombardment chamber. This pneumatic gene gun is eligible for a wide variety of cell types and makes the transformation efficient, stable and transient. The gene gun has a special mechanism by which it generates a gas shock wave and involves different rupture membrane at a certain pressure, ranging from 3.5Mpa to 10Mpa.
- EN: 60601 : 1
- EMD : 93/42/EEC
Material: Rice callus, PCAMBIA2301(11621bp, CaMV35S and GUS).
I. Hyper osmotic rice callus 4 hours before bombardment
II. Bio Gene BTI-GJ-1000 bombardment setting: Helium pressure 7.0Mpa, Target distance 60mm, Vacuum -0.095Mpa
I. GUS examination shows that:
Transient expression rate by Bio Gene BTI-GJ-1000 : 45%
Transient expression rate by Bio-Gene BTI-GG-1001
See Figure 1
II. Section of frozen tissue shows that:
The Particle is delivered into similar cellular layer after bombardment.
See Figure 2
- Pressure range: 3.5-10Mpa
- Max. Target area: 20 cm 2
- The diameter of the largest target: Φ 50mm
- Voltage: 220V
- Gas source: Helium or nitrogen
- N/W(KG):34 G/W:36
- Packing size: 57.5*47.5*32cm, 38*19*29.5cm