BioGene Fermenters can Grow almost any cell type With a wide range of vessels and impellers, including
Customized options, fermentation and bioreactor systems have the Capacity to grow virtually any cell type: whether it’s anaerobic or aerobic microbes, yeast, Insect, plant
Microbial cells have sturdy cell walls, and tend to clump together.
They withstand rigorous treatment, require large amounts of air, and need shearing impellers to break apart the clumps.
Mammalian cells have no cell walls and are fragile and sensitive.
Vessels for these cells come equipped with impellers that gently mix the media and minimize shearing.
- Temperature Control System
- Microprocessor Based Automated Heat Exchanger
- Automatic Agitation system
- Automatic Exhaust System
- The exhaust port on the Bio Gene fermenter is connected to atmosphere through Single Round 0.2 μ Sterilizable grade air filter and SS316L Housing.
- Cooling Condenser is provided to condense escaping moisture.
- Automatic ASME Compliance Bio Gene design in accordance with ASME Code. Filter area is designed as per the optimal pressure drop requirements.
- Automatic Aeration System
- Bio Gene Bio ferm air inlet port(s) of the Fermenter is connected to the air line through a sterilizable grade 0.2 μ air filters.
- Automatic pH / ORP Control System
- Automatic Redox Monitoring and Control
- Automatic Sampling System
- Automatic Harvesting System
- Automatic Nutrient Feed
- Foam Control System
- Touch Screen Automation and Control
- State of Art Control Cabinet
- Bio Gene uses state of Art Latest technology
- Bio Gene BioDASCADA Software
- Batch Recipe system, Batch creation & Initialization.
- Display of all parameters of all reactors in the same page with pop-up set point window
- Display of minute wise updates of all configured parameters, pH, DO, Flow, Pressure, Temp., RPM Error display and control page.
- Bio Gene BioFerm SCADA Software
- Bio Gene controller with variable 8 process microprocessor based advance controller
Alarms & Signals
- Culture medium temperature control (Range: 5 – 70oC; Precision 0.01oC)
- Culture medium pH control
- agitation rate control
- Foam level control
- Air Flow control
- High/Low temperature audiovisual signal
- High/Low pH audiovisual signal
- High/Low-agitation rate audiovisual signal
- High/Low-PO2 audiovisual signal
- High/Low-Air flow rate audiovisual signal
- High - Foam Audiovisual signal
- Top Driven Magnetic Drive
- Contamination free stirrer assembly with shaft
- Gaskets / O-rings Silicon & EPDM
- Height Adjustable 6 Blade Ruston Turbine-2 Nos.,
- Baffles 4 Nos. Removable Type Material SS316L
- The shaft is equipped with 3 No., 6 blade rushton turbine impeller with height adjustment facility and run on top driven agitator. Diameter of impeller is 0.40 times the inner diameter of the Fermenter. The agitator will include motor, agitator shaft with impellers, drive, mounting flange, variable frequency controller, Magnetic Drive/Double mechanical seal and driver support structure.
- Baffles are also provided to break the vortex formation. Agitator will be suitable for the range of specified operations and ambient conditions and for variable speed operations controlled by an independent control loop.
- MOC for the Impellers and shaft will be SS 316 L with a surface finish of Ra < 0.5 μ and fully electro polished.
- Bio Gene design in accordance with ASME Code. Filter area is designed as per the optimal pressure drop requirements.
- The SS 316 L filter housings are provided with tri-clamp connections to allow removal of the housings. The Sparger in the fermenter is connected to Air line.
- A pH sensor is provided on the fermenter for monitoring and controlling the pH of the contents of fermenter system. The Fermenter is provided with Peristaltic Pump for dosing acid and alkali stock solutions through food grade silicon tubing with autoclavable septum port assembly, responding to signal from online gel-filled pH sensor based on the pH set point. Addition of acid and alkali stock solutions achieves pH control in the fermenter.
- Sampling system consists of a zero dead leg manual sampling valve with Unique sampling assembly with zero dead leg.
- Peristaltic pumps (Positive displacement pump) are provided for nutrient feeding. Pumps can be controlled to feed nutrient based upon level based feeding, through automatic peristaltic pump feeding program module in SCADA and HMI.
- The Fermenter is provided with a Peristaltic Pump for dosing antifoam solution into the Fermenter, responding to signal from conductivity based SS-316L electrode foam sensor. Also gives automatic alarm.
- The Fermenter consists of a zero dead leg flush Bottom valve, provided on the bottom dish of the Fermenter vessel.
- The exhaust port on the Bio Gene fermenter is connected to atmosphere through Single Round 0.2 μ Sterilizable grade air filter and SS316L Housing.
- Cooling Condenser is provided to condense escaping moisture.
| Gross Volume | Working Vol |
5L | Up to 4L |
| Type |
Autoclavable |
| Aspect Ratio |
1:2 |
| Design Guidance |
Vessel |
| Design Code |
ASME Section VIII Div. BPE 2009 |
| Working pressure |
Full vacuum to 3 Kg/cm2 |
| Design Pressure |
Full Vacuum to 3.5 Kg/cm2 |
| Hydraulic Test Pressure |
Full Vacuum to 5.0 Kg/cm2 |
| Working Temperature |
10 to 140 deg. C |
| Design Temperature |
0 to 150 deg. C |
| Material of Construction |
| All Wetted Parts |
SS-316L / BOROSILICATE GLASS |
| Gaskets/ O-rings |
Silicon & EPDM |
| Finishing |
| Inner & External Surface |
220 grit Finished & double mirror polished Ra0.5m |
| Agitation |
| Drive |
Top Driven Magnetic Drive |
| Impellers |
6 Blade Ruston Turbine-2 Nos, |
| Baffles |
4 Nos. Removable |
| RPM |
0-1500 RPM |
| Motor type |
Standard DC motor |
| Ports |
| In Head Plate for |
Feeding (Acid, base, antifoam and nutrients) |
| Inoculation-Septum port |
| Air exhaust |
| Foam Sensor |
| pH Sensor |
| OD Sensor |
| DO Sensor |
| Sampling valve |
| Temperature sensor |
| Aeration |
| Designed for |
1.0 VVM |
| Sparger |
Ring type sparger with bottom perforations|Microsparger |
| Air filters |
0.2 micron filter| Exhaust-0.2 micron filter |
| Air Filters Operating Pressure |
Upto 3 Barg |
| Air Volume Measurement |
Variable Area Flow meter (Rotameter) (TWO SETS) |
| Air Exhaust |
Reflux condenser that can efficiently handle a volume of out flowing air |
| Air Flow control |
Automatic Control with Pneumatic control valve |
| Thermal Circuit |
| Temperature control |
With heat exchanger and circulation pump, temperature gauge, and all required valves to be connected to water supply. |
| Temperature Control |
| Type |
PID |
| Measurement Range |
0-150 deg C |
| Control Range |
05-80 deg C |
| Measurement precision |
+ 0.1 °C |
| Control precision |
+ 0.2 °C (up to 40° C) |
| Temp. sensor |
Pt.-100, 25mm, SS-316 port |
| RPM Control |
|
| RPM range |
0-1500 RPM |
| DC drive |
Electronic Frequency Control, Fully Automatic |
| Accuracy |
1 rpm |
| pH Control |
|
| pH Sensor |
Gel filled autoclavable probe |
| Measure Precision |
± 0.01 pH |
| Control Precision |
± 0.1 pH |
| Type |
Fully Automatic with Alarm Function |
| Range |
0-14 pH |
| Timer |
Acid and Base Timer |
| Peristaltic Pump |
2 |
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