Tissue Culture Chambers for Controlled Plant Propagation & Research

Made in India—Exported Worldwide to Biotech & Agri Institutions

Bio Gene Tissue Culture Chamber are engineered to create a clean, consistent environment for the in vitro development of plant tissues, callus cultures, and explants. Proudly manufactured and exported by Biotechnologies Inc., these chambers support precise control of temperature, light intensity, and humidity, essential for tissue growth and propagation. Ideal for use in biotechnology labs, agricultural research institutes, seed companies, and academic facilities, each unit features programmable lighting systems, air circulation for even distribution, and options for UV sterilization. Developed in India and relied on globally, Bio Gene chambers align with GMP protocols and scientific research standards.

International Standards Overview

Standards are divided into three main layers: Equipment Safety, Biological Containment, and Application-Specific Quality.

1. Equipment Safety & Performance (Global)

Foundational certifications ensuring the chamber is safe to operate and technically reliable:

  • ISO 9001:2015 & ISO 13485 — Standard for quality management in manufacturing and laboratory devices.
  • CE Marking (Europe) / UL & CSA (USA/Canada) — Mandatory for electrical safety, electromagnetic compatibility (EMC), and machinery safety.
  • IEC 61010-1 — Specific safety requirement for electrical laboratory equipment.
  • ICH Q1A (R2) — For chambers used in stability testing (common in pharma and seed research); requires strict temperature (±2°C) and humidity (±5% RH) tolerances.

2. Biological Containment (Biosafety)

Critical for insect rearing to prevent the escape of exotic or infected species.

  • Arthropod Containment Levels (ACL 1–4) — Global benchmark (originally from USA's CDC/NIH) for insect labs.
    • ACL-1 — For indigenous or non-viable exotic insects (basic pest control, screened cages).
    • ACL-2 — For insects infected with BSL-2 agents or transgenic (GM) insects. Requires sealed rooms, double-door entries, and strict waste decontamination (autoclaving).
    • ACL-3/4 — For high-risk pathogens (e.g., Zika, Malaria). Requires negative pressure and HEPA filtration.
  • Plant Biosafety (BL1-P to BL4-P) — Similar levels for plant research involving recombinant DNA or exotic pathogens.

3. Country-Specific Guidelines

  • USA
    • Compliance with BMBL 6th Edition
    • USDA-APHIS PPQ 526 permits for exotic pests
    • 21 CFR Part 11 required for electronic data logging in regulated industries
  • European Union
    • Machinery Directive (2006/42/EC)
    • Directive 2009/41/EC for the contained use of genetically modified organisms
  • India
    • DBT Biosafety Guidelines for GM research
    • IBSC (Institutional Biosafety Committee) approval required
    • SAMAR Level-3 — highest maturity mark for defense and high-precision engineering manufacturing
  • Australia/NZ
    • AS/NZS 2243.3 (Physical Containment levels PC1–PC4)

The BioGene Advantage

  • SAMAR Level-3 certification already meets "Advanced/Mature" requirements for high-precision systems.
  • For global B2B clients: chambers can be positioned as meeting ACL-2 containment and ICH stability standards as a baseline.

Applications

Bio Gene Tissue Culture Chamber are widely used in Hospitals Medical Facilities & Laboratories, Industrial Manufacturing, Research Centers, and Universities.

Bio Gene Tissue Culture Chamber and rooms are specifically designed to maximize research area and to provide uniform Environmental conditions. They are ideal for tissue culture work and genetic experiments.

Feature

Tissue culture area total on four- six tiers with 95μmoles/m²/s P.A.R. of lighting Ideal for tissue culture requiring upward airflow to reduce condensation Four tiers of single or double row shelving maximizing tissue culture area Through the shelf airflow reduces condensation Small footprint maximizing floor space in the lab

  • Bio Gene Tissue Culture Chamber is most suitable for circulation free environment to eliminate cross contamination in sterile techniques involving culture growth, tissue staining and virus harvesting.
  • The convenient working height and ample working space provides working ease for uses while media preparation.
  • The Inner Chamber  is made of thick Stainless Steel Sheet304 grade
  • Tray surface will be of 3mm thick hylem Sheet.
  • Light Facility of LED Blue, Red & Far Red or fluorescent lights.
  • Shelves :shelf to shelf distance Adjustable
  • Temperature Range: Ambient-5ºC - 40ºC  
  • Minimum heating effect/heat dissipation.
  • No Flickering effect.
  • Energy saver with individual switching arrangement in each shelf.
  • Outer Body made out of Thick CRCA Sheet duly powder coated.
  • Wheel/Castor
  • Provision for connecting the Tissue Culture Chamber to photoperiodic Timer/Controller output
  • Input 200-400 VAC, 50 Hz, Single phase, 
  • Bio Gene  tissue culture chamber specifically designed with an open channel floor on each shelf delivering upward airflow to minimize or eliminate condensation in the tissue culture dishes.
  • Uniform control of temperature, lighting using fluorescent lamps, and adjustable airflow
  • RH up to 95%
  • The front door is made of thick transparent Plexiglas and has arrangement for keeping the door open if desired.
  • The units are fitted with one U.V germicidal light and fluorescent LED light for Sterilization & illuminator respectively.

Control

HUMIDITY CONTROL
Humidity control Bio gene uses a highly accurate Humidity sensor. This is linked to a microprocessor which controls the switching of the Humidifier. MICROPROCESSOR BASED DIGITAL HUMIDITY INDIACATOR – cum – CONTROLLER to control humidity form 40% to 95% at cool temperatures. Light intensity can be adjusted manually according to requirements.

HUMIDITY RANGE
90% + 5% (as per the graph attached)/ (As per the temperature Requirements).

HUMIDIFICATION
A low-pressure droplet free vapour boiler using direct vaporization system is used. Water Reservoir is provided at the side of the chamber with a 10 x 8mm PU tube quick connector and water level is automatically controlled through a water-in solenoid valve. The wick tank water level is automatically maintained through a water solenoid valve and an electrical float switch. The de-mineralized water or single distilled water supply to the humidity inlet having a resistance measurement of approximately 50,000 – 200,000 Ohm/cm is provided with the unit.

DEHUMIDIFICATION
Refrigeration based de-humidification system is used. The desired level of lower humidity will be achieved by maintaining the precise dew point temperature. The output will automatically be activated based on the set point as well as in ramp up mode after a low temperature cycle.

ILLUMINATION: 
Bio gene units are provided with door operated illumination system comprising of fluorescent lights. The unit is designed to provide 100-150 Micro mol light intensity. Light Bank System (Option) programmable control from Three side, control from 15,000 to 25,000 Lux.

HUMIDITY SENSITIVITY:
Humidity is controlled by mean of an electronic humidity controller cum indicator with an accuracy of +5%.

TEMPERATURE CONTROL:
The temperature inside our temperature and humidity control cabinets is controlled through programmable micro-processor based temperature controller cum indicator.

TEMPERATURE RANGE:
Temperature ranges of our standard models are 0-40°C. Range 4°C to 40°C all lights off, 10°C to 40°C all lights on (extended temperature options are available).  However we have the capacity to modify the same to suit the individual specialized requirements of our customers.

TEMPERATURE SENSITIVITY:
Temperature inside our temperature and humidity control cabinets is controlled with a sensitivity of +1° c or better.

FRONT PANEL:
Front panel of our units comprises of on/off switches heating, cooling and mains indicator lamps, temperature controllers. Lux controller,

Construction & Features of BioGene Tissue Culture Chamber

These are sturdy, double-walled units with an inner chamber made of stainless steel, designed for reliable performance and uniform environmental conditions in tissue culture applications.

Interior

The interior is made of stainless steel and is provided with internal lighting for convenient observation and monitoring.

Exterior

The exterior is constructed from powder-coated CRCA steel for durability and corrosion resistance.

Heating System

Tubular heating elements are positioned in the ribs, bottom, and sides of the chamber to ensure uniform heat distribution.

Adjustable Racks

The inner chamber is provided with ribs for adjusting perforated shelves to convenient heights. The shelves are made of thick SS 304-grade stainless steel.

Door Gasket

A synthetic neoprene rubber door gasket is fitted to all BioGene units to provide a secure seal.

Air Ventilators

Air ventilators are provided on the sides of the unit to support air exchange as required by the chamber design.

Doors

The double-walled outer door is made of thick stainless steel, with an inner wall constructed from SS 304-grade stainless steel.

Inner Door

The inner door is made of thick glass or acrylic sheet, depending on customer requirements and application needs.

Lock and Key

The door is provided with a lock-and-key arrangement for secure and convenient operation.

Air Circulation Fan

A blower fan is installed at the rear wall of the chamber to promote uniform air circulation and maintain consistent temperature distribution throughout the chamber.

Electrical Requirements

The unit operates on 220–240 V AC, 50 Hz power supply. The stated power consumption is approximately 1,800–2,000 W during heating, subject to model specifications. Specially designed fans help maintain uniform temperature under suitable tropical and dry operating conditions.

Over-Temperature Protection

A safety thermostat automatically cuts off heating when the temperature rises approximately 10°C above the set value. It provides additional protection in the event of a faulty sensor, sensor failure, or switching-element malfunction.

Insulation

The gap between the inner and outer walls is filled with high-grade polyurethane foam (PUF) insulation to improve thermal efficiency and temperature stability in the BioGene microprocessor-based Tissue Culture Chamber.

Tissue Culture Chamber Import & Export Services Worldwide

BioGene Tissue Culture Chamber import and export services are available for international markets, including America, the USA, Germany, Japan, China, Mexico, Vietnam, South Korea, Canada, the UK, France, Italy, Spain, the Netherlands, Australia, Russia, Kazakhstan, Pakistan, Bangladesh, the Philippines, Indonesia, Malaysia, Thailand, Singapore, Saudi Arabia, the UAE, South Africa, Turkey, Brazil, and Poland.

We support international shipping, export documentation, customs clearance coordination, and applicable regulatory compliance requirements to help customers source laboratory and tissue culture equipment worldwide.

Specification

DISCRIPTION BTI-TCCH-170
TEMPERATURE RANGE 0 to 40 °C
TEMPERATURE STABILITY < +1°C
HOUSING MATERIAL CRC / Mild Steel Sheet duly Powder Coated
SAFETY FUNCTION Water Level & Low water level cut off alarm (On customer request)
OPTIONAL RS 232 Communication Interface
DUTY CYCLE Continuous
INTERIOR S.S.304 Polished Sheets
HUMIDITY RANGE 50 %{Constant}RH + 3% 25% at 25°C
COMPRESSOR Kirloskar/ Tecumseh As per model requirement (For Cooling below Ambient)
PRECAUTION Don’t use the Instrument without water
DIMENSION 900 x 600 mm (without Glove port and Gauntlets)

A Tissue Culture Chamber is a controlled environment used to grow and multiply plant or animal tissue under sterile conditions. It regulates temperature, humidity, light intensity, and photoperiod so that cells, tissues, or plantlets can develop without contamination from bacteria, fungi, or other external organisms. It is widely used in plant micropropagation, research laboratories, pharmaceutical development, and agricultural biotechnology.

Sterility is maintained through a combination of HEPA filtration, UV sterilization, and strict handling protocols. Before placing culture vessels inside, the chamber and work surfaces should be wiped with 70% ethanol or an approved disinfectant. Regular cleaning schedules, along with periodic UV exposure cycles when the chamber is not in use, help prevent microbial buildup. Operators should also follow proper gowning and hand hygiene practices before accessing the chamber.

Most tissue culture applications require a temperature range of 22°C to 26°C and relative humidity between 50% and 70%, though exact settings depend on the species or cell line being cultured. It is best to consult the specific protocol for your plant or tissue type, as some cultures may need higher humidity or slightly cooler temperatures to prevent stress or premature growth.

Routine calibration of temperature and humidity sensors is recommended every three to six months to ensure accuracy. Light fixtures, especially LED or fluorescent grow lights, should be checked periodically for output degradation, as reduced intensity can affect photosynthesis and growth rates. Filters (such as HEPA filters) should be inspected and replaced according to the manufacturer's guidelines, typically once or twice a year depending on usage frequency.

If contamination is detected, remove the affected culture vessel immediately to prevent it from spreading to other samples. Disinfect the chamber thoroughly, including shelves, walls, and any equipment that may have come into contact with the contaminated material. Review your sterile technique and chamber maintenance routine to identify the possible source, whether it is airflow, improper sealing of vessels, or lapses in handling procedure, and adjust practices accordingly going forward.
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